Title [pFind] modification doesn't work
Priority question Status finished
Superseder Nosy List DZ, kfwang
Assigned To kfwang Keywords pFind

Created on 2021-04-18.13:46:34 by DZ, last changed by kfwang.

File name Uploaded Type Edit Remove
图1.png DZ, 2021-04-19.12:33:55 image/png
msg50 (view) Author: kfwang Date: 2021-04-20.13:51:31

For the identification results, when amino acid C appears, whether the modification 
appears or not is random. In fact, pFind considers all possibilities of whether the 
modification occurs, and all possible outcomes are involved in the identification.
Therefore, this modification is considered for all amino acids C, but the end result is 
likely to be that only part of C is modified.

Wang kaifei
msg44 (view) Author: DZ Date: 2021-04-19.12:33:55
Maybe I forgot to send the picture, so my question may be misunderstood. I think 
the protein in pBuild may not display all carbamidomethyl[C]. So I don't know 
whether every C is considered.
msg40 (view) Author: kfwang Date: 2021-04-19.00:09:45

At present, the definitions of variable modification and fixed modification of pFind 
are very similar. Fixed modification should also consider all permutations and 
combinations of the modification. The only difference is that the max number of 
variable modifications per peptide is 3, but fixed modifications don't have this 

Wang kaifei
msg39 (view) Author: DZ Date: 2021-04-18.13:46:34
In pFind, when I add modification of Carbamidomethyl[C], however in pBuild I find 
that the protein was modified in only one place.
Date User Action Args
2021-04-26 19:27:01kfwangsetstatus: chatting -> finished
2021-04-20 13:51:35kfwangsetmessages: + msg50
2021-04-19 12:34:00DZsetfiles: + 图1.png
messages: + msg44
2021-04-19 00:09:48kfwangsetmessages: + msg40
status: unread -> chatting
2021-04-18 17:47:30ctarnsetassignedto: kfwang
nosy: + kfwang
2021-04-18 13:46:36DZcreate