Hi pLink team,
I recently started a new project to identify SUMO-1 modification in a
purified protein. Unfortunately, I am not able to understand how to but in
the parameters in pConfig for SUMO-1 linker. could you please help me with
this? My protein of interest is SUMOlyated (SUMO-1), I do protein digestion
with trypsin followed by chymotrypsin, so I end up with a shorter sequence
QEQTGG attached to K of protein. I have tired using this sequence as
variable modification, but I want to put it as linker. Is there any
parameters that I need to change? Do you have a tutorial for SUMO
identification.
Looking forward to your help.
Regards,
Sheon
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