Hi. Thank you. According to the error message, pFind tried to analyze HCD&IT (ion
trap) MS data but could not find any HCD&IT scan extracted from the .raw file. If
you really want to do this, please check the .raw file or try to convert it manually
to .mgf files using tools like MsConvert. Otherwise, please check the settings of
pFind and try again.
I have four raw runs for four different samples that were run on an orbitrap
fusion. We are looking for a specific Trypstic peptide that may be highly
modified. The Fasta only contains the sequence for the protein of interest.
Whenever I start the seach on pFind, there is an unexpected error:
[pFind] ALERT: Invalid_msmspath1_Path: