Title [pLink] some.mgf files are not compatible with pLink
Priority question Status chatting
Superseder Nosy List LauraM, ctarn
Assigned To ctarn Keywords

Created on 2022-05-17.20:46:50 by LauraM, last changed by ctarn.

File name Uploaded Type Edit Remove
image.png LauraM, 2022-05-17.20:46:50 image/png
msg236 (view) Author: ctarn Date: 2022-05-22.15:57:06
Hi, Thanks for reaching out. The message shows that the .mgf file is not compatible 
with pLink. Could you please open the file with a text editor and show us what it 
looks like?
msg234 (view) Author: LauraM Date: 2022-05-18.01:40:22
This is the error I get if I run the searcher in command line mode

PS C:\pFindStudio\pLink\2.3.11\bin> .\searcher.exe "C:\Users\laura\Documents\pLink\pLink_task_2022.\pLink_task_2022."
[pLink] Welcome to use pLink v2.3.11, it will be expired on 20250110
[pLink] Search Engine initializing...
[pLink] Generating reverse database...
[pLink] Search identifier: NUXKL
[pLink] Search Engine is ready to search.

[pLink] Start searching C:\Users\laura\Desktop\MassSpecDSS\May2022\XL-IP\220504kw_LauraXLIP_M3_S3-A11_1_9302.mgf, Labeling None
         at MGFInput::checkLegalityAndCount() loss information of title, charge or peptide m/z.
         at MGFInput::checkLegalityAndCount() there are problems in the MGF file.
         at MGFInput::startLoad() OpenInFile: C:\Users\laura\Desktop\MassSpecDSS\May2022\XL-IP\220504kw_LauraXLIP_M3_S3-A11_1_9302.mgf failed.
         at Searcher::search() search failed!
         at Searcher::main() caught an exception.
msg233 (view) Author: LauraM Date: 2022-05-17.20:46:50
Hi pLink Team,
I would like to ask you why pLink works perfectly with some samples but it doesn't 
work with others; it can run forever without generating any results or messages
(I use DSS as crosslinker).
Date User Action Args
2022-05-22 16:07:03ctarnsetassignedto: ctarn
2022-05-22 16:06:50ctarnsettitle: No results -> [pLink] some.mgf files are not compatible with pLink
2022-05-22 15:57:07ctarnsetmessages: + msg236
nosy: + ctarn
2022-05-18 01:40:25LauraMsetpriority: urgent -> question
messages: + msg234
status: unread -> chatting
2022-05-17 20:46:55LauraMcreate