Issue 135: [pLink] pLink stops after "Search Engine is ready to search" - pFind Forum

Title	[pLink] pLink stops after "Search Engine is ready to search"
Priority	urgent	Status	finished
Superseder		Nosy List	dko22, pzmao
Assigned To	pzmao	Keywords	plink

Created on 2023-07-12.16:24:04 by dko22, last changed by pzmao.

Files
File name	Uploaded	Type	Edit	Remove
1.png	dko22, 2023-07-12.16:24:20	image/png
2.png	dko22, 2023-07-12.16:24:31	image/png
2023-06-28_BSA_XL_DSS.plink	dko22, 2023-07-12.16:26:18	application/octet-stream
3.png	dko22, 2023-07-12.16:24:42	image/png
T7163_BSA_XL_DSS_50_5uL_S3-A11_1_6715_6.0.434.mgf	dko22, 2023-07-12.16:26:48	application/octet-stream
cmd.png	dko22, 2023-07-12.16:25:34	image/png
linker.png	dko22, 2023-07-12.16:29:22	image/png

Messages
msg359 (view)	Author: pzmao	Date: 2023-07-12.19:50:33
I have replied to you by email at support@pfind.org.
msg358 (view)	Author: dko22	Date: 2023-07-12.16:28:23
If I run the plink-file in the cmd it is displayed to me, that there are problems in the MGF-file, but I don't know why. When I open the MGF-file in the editor, it look quite normal to me.
msg356 (view)	Author: dko22	Date: 2023-07-12.16:24:04
Dear pLink team, I've made a test reaction for crosslinking. I used a protein (P02769 · ALBU_BOVIN) and the crosslinker DSS (Disuccinimidyl suberate). I've made a normal reduction with TCEP and alkylation with MMTS, followed by a tryptic digest. After measuring the samples on our mass spectrometer (timsTOF Pro) I got the ".mgf"-files and wanted to do the data analysis in pLink2. I adjusted the settings in pLink2, set the crosslinker, the database and also the modification. If I start the run the program starts and stops at "search engine is ready to search" and doesn't continue. Thanks for your help in advance. Dominik

History
Date	User	Action	Args
2023-07-12 19:51:52	pzmao	set	status: processing -> finished
2023-07-12 19:50:35	pzmao	set	status: chatting -> processing assignedto: pzmao keyword: + plink messages: + msg359 nosy: + pzmao
2023-07-12 16:29:22	dko22	set	files: + linker.png
2023-07-12 16:28:32	dko22	set	messages: - msg357
2023-07-12 16:28:24	dko22	set	messages: + msg358
2023-07-12 16:26:48	dko22	set	files: + T7163_BSA_XL_DSS_50_5uL_S3-A11_1_6715_6.0.434.mgf
2023-07-12 16:26:20	dko22	set	files: + 2023-06-28_BSA_XL_DSS.plink messages: + msg357 status: unread -> chatting
2023-07-12 16:25:34	dko22	set	files: + cmd.png
2023-07-12 16:24:42	dko22	set	files: + 3.png
2023-07-12 16:24:31	dko22	set	files: + 2.png
2023-07-12 16:24:20	dko22	set	files: + 1.png
2023-07-12 16:24:05	dko22	create