Title [pLink] pLink stops after "Search Engine is ready to search"
Priority urgent Status finished
Superseder Nosy List dko22, pzmao
Assigned To pzmao Keywords plink

Created on 2023-07-12.16:24:04 by dko22, last changed by pzmao.

File name Uploaded Type Edit Remove
1.png dko22, 2023-07-12.16:24:20 image/png
2.png dko22, 2023-07-12.16:24:31 image/png
2023-06-28_BSA_XL_DSS.plink dko22, 2023-07-12.16:26:18 application/octet-stream
3.png dko22, 2023-07-12.16:24:42 image/png
T7163_BSA_XL_DSS_50_5uL_S3-A11_1_6715_6.0.434.mgf dko22, 2023-07-12.16:26:48 application/octet-stream
cmd.png dko22, 2023-07-12.16:25:34 image/png
linker.png dko22, 2023-07-12.16:29:22 image/png
msg359 (view) Author: pzmao Date: 2023-07-12.19:50:33
I have replied to you by email at
msg358 (view) Author: dko22 Date: 2023-07-12.16:28:23
If I run the plink-file in the cmd it is displayed to me, that there are problems in the MGF-file, but I don't know why. When I open the MGF-file in the editor, it look quite normal to me.
msg356 (view) Author: dko22 Date: 2023-07-12.16:24:04
Dear pLink team,

I've made a test reaction for crosslinking.
I used a protein (P02769 ยท ALBU_BOVIN) and the crosslinker DSS (Disuccinimidyl suberate). I've made a normal reduction with TCEP and alkylation with MMTS, followed by a tryptic digest. 

After measuring the samples on our mass spectrometer (timsTOF Pro) I got the ".mgf"-files and wanted to do the data analysis in pLink2.
I adjusted the settings in pLink2, set the crosslinker, the database and also the modification.

If I start the run the program starts and stops at "search engine is ready to search" and doesn't continue.

Thanks for your help in advance.
Date User Action Args
2023-07-12 19:51:52pzmaosetstatus: processing -> finished
2023-07-12 19:50:35pzmaosetstatus: chatting -> processing
assignedto: pzmao
keyword: + plink
messages: + msg359
nosy: + pzmao
2023-07-12 16:29:22dko22setfiles: + linker.png
2023-07-12 16:28:32dko22setmessages: - msg357
2023-07-12 16:28:24dko22setmessages: + msg358
2023-07-12 16:26:48dko22setfiles: + T7163_BSA_XL_DSS_50_5uL_S3-A11_1_6715_6.0.434.mgf
2023-07-12 16:26:20dko22setfiles: + 2023-06-28_BSA_XL_DSS.plink
messages: + msg357
status: unread -> chatting
2023-07-12 16:25:34dko22setfiles: + cmd.png
2023-07-12 16:24:42dko22setfiles: + 3.png
2023-07-12 16:24:31dko22setfiles: + 2.png
2023-07-12 16:24:20dko22setfiles: + 1.png
2023-07-12 16:24:05dko22create