Dear pLink team,
I've made a test reaction for crosslinking.
I used a protein (P02769 ยท ALBU_BOVIN) and the crosslinker DSS (Disuccinimidyl suberate). I've made a normal reduction with TCEP and alkylation with MMTS, followed by a tryptic digest.
After measuring the samples on our mass spectrometer (timsTOF Pro) I got the ".mgf"-files and wanted to do the data analysis in pLink2.
I adjusted the settings in pLink2, set the crosslinker, the database and also the modification.
If I start the run the program starts and stops at "search engine is ready to search" and doesn't continue.
Thanks for your help in advance.
Dominik |