Message58

Author SheonSamji
Recipients SheonSamji
Date 2021-05-06.01:25:42
Content
Hi pLink team,

I recently started a new project to identify SUMO-1 modification in a 
purified protein. Unfortunately, I am not able to understand how to but in 
the parameters in pConfig for SUMO-1 linker. could you please help me with 
this? My protein of interest is SUMOlyated (SUMO-1), I do protein digestion 
with trypsin followed by chymotrypsin, so I end up with a shorter sequence 
QEQTGG attached to K of protein. I have tired using this sequence as 
variable modification, but I want to put it as linker. Is there any 
parameters that I need to change? Do you have a tutorial for SUMO 
identification.
Looking forward to your help.

Regards,
Sheon
History
Date User Action Args
2021-05-06 01:25:45SheonSamjisetmessageid: <1620235542.629923.FCTFZ57G6SASDYWG.issue19@pfind.org>
2021-05-06 01:25:45SheonSamjisetrecipients: + SheonSamji
2021-05-06 01:25:45SheonSamjilinkissue19 messages
2021-05-06 01:25:45SheonSamjicreate